Luciferase Assay System Protocol - promega.co.uk.

A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene.

Luciferase Essay

Luciferase assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full range of luciferase assay products and tools for your research.

Why You Don't Need to Select a Wavelength for a Luciferase.

Firefly luciferase assays (SCT151, SCT154, LUC1, LUCASSY-RO) are designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples.Luciferase Assays Thermo Scientific Pierce Luciferase Reporter Assays allow you to investigate promoter activity by measuring light output from luciferase enzyme that is expressed under the control of your promoter of interest.The Luciferase Assay System incorporates coenzyme A (CoA) for improved kinetics, allowing greater enzymatic turnover and resulting in increased light intensity that is nearly constant for at least 1 minute. The Luciferase Assay System yields linear results over at least eight orders of magnitude.


Several commercially luciferase reporter assay kits are available that contain an expression vector containing the luciferase gene. A luciferase reporter assay is usually performed to investigate the strength and regulation of a promoter.Luciferase assays allow for the study of transcriptional gene expression, virus life cycles, and cell viability, making them significant tools for drug development in reporter gene, cytotoxicity, and cell proliferation applications, as well as general kinase activity monitoring.

Luciferase Essay

The Ready-To-Glow Secreted Luciferase Reporter Assay is based on the secreted Metridia luciferase reporter. It combines the advantages of a live-cell assay with the sensitivity of an enzyme-based assay.

Luciferase Essay

An important advantage of the new test, called a Luciferase Immunoprecipitation Systems (LIPS) assay, is that it is less complicated and easier to perform than other assays now available. Moreover.

Luciferase Essay

Multicolored Luciferase Reporter assay: This assay is a one-step method for simultaneous monitoring of many extracts. It is useful for large-scale data interpretation in lesser time and amount of sample. Different beetle luciferases are utilized to emit different colors using modified luciferases and all the emissions can be detected separately.

Luciferase Immunoprecipitation System (LIPS) assay is a.

Luciferase Essay

Gaussia Luciferase (GLuc) is a recently discovered, naturally secreted protein by the copepod, Gaussia princeps (1). At 19kD, it is the smallest known luciferase and its catalytic properties make it one of the “brightest” known (2). Additionally, multiple disulfide bonds contribute to the protein stability at elevated temperatures. These properties make it an ideal reporter gene, either as.

Luciferase Essay

Lucia luciferase is optimized for strong bioluminescent activity: 1000-fold higher signal compared to Firefly and Renilla luciferases. Lucia is ideal for reporter cell based assays. Activity can be rapidly monitored in supernatant.

Luciferase Essay

The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary.

Luciferase Essay

Combined with choices for traditional Firefly and Renilla luciferase assay kits to provide compatibility with multiple systems, as well as superior quality luciferase reporter clones for miRNA targets, promoters, and transcriptional response elements, GeneCopoeia's luciferase products provide you with a wealth of tools for luminescence reporter analysis.

Luciferase Essay

Assay Principle. The reaction is catalyzed by the enzyme luciferase obtained from the firefly (Photinus pyralis). The MgATP 2-converts the luciferin into a form which is capable of being catalytically oxidized by the luciferase in a high quantum yield chemiluminescent reaction, according to the following equation.

ATP Bioluminescence Assay to quantify Cell Cytotoxicity.

Luciferase Essay

Luciferase reporter assay To analyze SOSTDC1 promoter activity, the full-length SOSTDC1 promoter regions were cloned into the reporter vector (Promega Corporation, USA).. K1 cells were transfected with either vectors containing the SOSTDC1 promoter or control vectors.

Luciferase Essay

Luciferase Reporter Assay Substrate Kit - Firefly (ab228530) provides a fast, simple, and homogeneous bioluminescence assay for the quantification of luciferase activity in live cells and cell extracts. This assay is based on firefly luciferase, a monomeric 61 kD enzyme that catalyses a two-step oxidation of luciferin, which yields light at 560 nm. The assay can be performed in a convenient 96.

Luciferase Essay

The Renilla Luciferase Assay utilizes a special buffer formulation designed to yield reliable, linear measurements of Renilla luciferase activity with minimal autoluminescence background and superior sensitivity. This is a flash-type luminescence assay that requires signal to be measured immediately after adding working solution to samples. The luminescence signal decays over the course of.

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